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VILBER GmbH
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Lonza
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Nikon
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Millipore
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Agilent technologies
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Vilber Lourmat
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VILBER GmbH
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Philips Healthcare
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Philips Healthcare
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Beiersdorf Inc
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RUVBL1 Antibody raised in Rabbit validated in WB IHC P Flow in Human
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Image Search Results
Journal: The Journal of investigative dermatology
Article Title: Smad4 loss in mouse keratinocytes leads to increased susceptibility to UV carcinogenesis with reduced Ercc1-mediated DNA repair
doi: 10.1038/jid.2013.213
Figure Lengend Snippet: (a) Immunofluorescence staining of p-H2AX in UV-irradiated mouse skin and tumors. Keratin 14 was used as counterstain. Scale bar is 100μm. (b) Quantification of p-H2AX staining, n=6, 8 and 4 for WT skin, Smad4 −/− skin, Smad4 −/− tumors, respectively. (c) Alkaline comet assay using WT and Smad4 −/− keratinocytes. (d) Quantification of % DNA in comet tail. n=44. (e) CPD levels in UVB-irradiated WT and Smad4 −/− keratinocytes was measured by ELISA. CPD level in Smad4 −/− keratinocytes at 10 minutes was set at 100%. P ≥ 0.1 at 5, 10 and 30 minutes, respectively. *denotes p ≤ 0.0002, and ** denotes p ≤ 0.003, n=3. Error bars are S.E.M. (all panels).
Article Snippet: Keratinocytes in culture were irradiated with 60 mJ/cm 2 of
Techniques: Immunofluorescence, Staining, Irradiation, Alkaline Single Cell Gel Electrophoresis, Enzyme-linked Immunosorbent Assay
Journal: The Journal of investigative dermatology
Article Title: Smad4 loss in mouse keratinocytes leads to increased susceptibility to UV carcinogenesis with reduced Ercc1-mediated DNA repair
doi: 10.1038/jid.2013.213
Figure Lengend Snippet: (a) Snai1 mRNA level in UV-irradiated keratinocytes, the level in untreated WT keratinocytes was set at 1. (b) Snai1 mRNA level in UV-irradiated mice skin, the level in UV-irradiated WT skin was set at 1, n=4 for WT , n=7 for Smad4 −/− . * denotes p= 0.027 (c) Ercc1 mRNA level in human Snai1 -transfected keratinocytes, the level in empty vector transfected WT keratinocytes was set at 1. ** denotes p≤ 0.002. (d) Snail Chromatin Immunoprecipitation from WT and Smad4 −/− keratinocyte extracts with/without UV irradiation. * p<0.03, ** p< 0.005. (e) CPD level in UVB-irradiated Snai1 transfected keratinocytes *p = 5.09E −5 , ** p=0.005, *** p=0.01. (f) CPD level in UVB-irradiated Ercc1 transfected keratinocytes, *=0.026, **=0.046, *** p=0.10, n=3. Error bars: S.E.M.
Article Snippet: Keratinocytes in culture were irradiated with 60 mJ/cm 2 of
Techniques: Irradiation, Transfection, Plasmid Preparation, Chromatin Immunoprecipitation