digital uvb meter 6.0 Search Results


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VILBER GmbH uv irradiation
Uv Irradiation, supplied by VILBER GmbH, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Lonza culture medium
(a) Immunofluorescence staining of p-H2AX in UV-irradiated mouse skin and tumors. Keratin 14 was used as counterstain. Scale bar is 100μm. (b) Quantification of p-H2AX staining, n=6, 8 and 4 for WT skin, Smad4 −/− skin, Smad4 −/− tumors, respectively. (c) Alkaline comet assay using WT and Smad4 <t>−/−</t> <t>keratinocytes.</t> (d) Quantification of % DNA in comet tail. n=44. (e) CPD levels in <t>UVB-irradiated</t> WT and Smad4 −/− keratinocytes was measured by ELISA. CPD level in Smad4 −/− keratinocytes at 10 minutes was set at 100%. P ≥ 0.1 at 5, 10 and 30 minutes, respectively. *denotes p ≤ 0.0002, and ** denotes p ≤ 0.003, n=3. Error bars are S.E.M. (all panels).
Culture Medium, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Nikon eclipse ts 100 microscope
(a) Immunofluorescence staining of p-H2AX in UV-irradiated mouse skin and tumors. Keratin 14 was used as counterstain. Scale bar is 100μm. (b) Quantification of p-H2AX staining, n=6, 8 and 4 for WT skin, Smad4 −/− skin, Smad4 −/− tumors, respectively. (c) Alkaline comet assay using WT and Smad4 <t>−/−</t> <t>keratinocytes.</t> (d) Quantification of % DNA in comet tail. n=44. (e) CPD levels in <t>UVB-irradiated</t> WT and Smad4 −/− keratinocytes was measured by ELISA. CPD level in Smad4 −/− keratinocytes at 10 minutes was set at 100%. P ≥ 0.1 at 5, 10 and 30 minutes, respectively. *denotes p ≤ 0.0002, and ** denotes p ≤ 0.003, n=3. Error bars are S.E.M. (all panels).
Eclipse Ts 100 Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Millipore solar simulator (suv1000, 290–400
(a) Immunofluorescence staining of p-H2AX in UV-irradiated mouse skin and tumors. Keratin 14 was used as counterstain. Scale bar is 100μm. (b) Quantification of p-H2AX staining, n=6, 8 and 4 for WT skin, Smad4 −/− skin, Smad4 −/− tumors, respectively. (c) Alkaline comet assay using WT and Smad4 <t>−/−</t> <t>keratinocytes.</t> (d) Quantification of % DNA in comet tail. n=44. (e) CPD levels in <t>UVB-irradiated</t> WT and Smad4 −/− keratinocytes was measured by ELISA. CPD level in Smad4 −/− keratinocytes at 10 minutes was set at 100%. P ≥ 0.1 at 5, 10 and 30 minutes, respectively. *denotes p ≤ 0.0002, and ** denotes p ≤ 0.003, n=3. Error bars are S.E.M. (all panels).
Solar Simulator (Suv1000, 290–400, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Agilent technologies stratalinker uv crosslinker
(a) Immunofluorescence staining of p-H2AX in UV-irradiated mouse skin and tumors. Keratin 14 was used as counterstain. Scale bar is 100μm. (b) Quantification of p-H2AX staining, n=6, 8 and 4 for WT skin, Smad4 −/− skin, Smad4 −/− tumors, respectively. (c) Alkaline comet assay using WT and Smad4 <t>−/−</t> <t>keratinocytes.</t> (d) Quantification of % DNA in comet tail. n=44. (e) CPD levels in <t>UVB-irradiated</t> WT and Smad4 −/− keratinocytes was measured by ELISA. CPD level in Smad4 −/− keratinocytes at 10 minutes was set at 100%. P ≥ 0.1 at 5, 10 and 30 minutes, respectively. *denotes p ≤ 0.0002, and ** denotes p ≤ 0.003, n=3. Error bars are S.E.M. (all panels).
Stratalinker Uv Crosslinker, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Vilber Lourmat uvb lamp
(a) Immunofluorescence staining of p-H2AX in UV-irradiated mouse skin and tumors. Keratin 14 was used as counterstain. Scale bar is 100μm. (b) Quantification of p-H2AX staining, n=6, 8 and 4 for WT skin, Smad4 −/− skin, Smad4 −/− tumors, respectively. (c) Alkaline comet assay using WT and Smad4 <t>−/−</t> <t>keratinocytes.</t> (d) Quantification of % DNA in comet tail. n=44. (e) CPD levels in <t>UVB-irradiated</t> WT and Smad4 −/− keratinocytes was measured by ELISA. CPD level in Smad4 −/− keratinocytes at 10 minutes was set at 100%. P ≥ 0.1 at 5, 10 and 30 minutes, respectively. *denotes p ≤ 0.0002, and ** denotes p ≤ 0.003, n=3. Error bars are S.E.M. (all panels).
Uvb Lamp, supplied by Vilber Lourmat, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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VILBER GmbH uvb source
(a) Immunofluorescence staining of p-H2AX in UV-irradiated mouse skin and tumors. Keratin 14 was used as counterstain. Scale bar is 100μm. (b) Quantification of p-H2AX staining, n=6, 8 and 4 for WT skin, Smad4 −/− skin, Smad4 −/− tumors, respectively. (c) Alkaline comet assay using WT and Smad4 <t>−/−</t> <t>keratinocytes.</t> (d) Quantification of % DNA in comet tail. n=44. (e) CPD levels in <t>UVB-irradiated</t> WT and Smad4 −/− keratinocytes was measured by ELISA. CPD level in Smad4 −/− keratinocytes at 10 minutes was set at 100%. P ≥ 0.1 at 5, 10 and 30 minutes, respectively. *denotes p ≤ 0.0002, and ** denotes p ≤ 0.003, n=3. Error bars are S.E.M. (all panels).
Uvb Source, supplied by VILBER GmbH, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Philips Healthcare tl12 fluorescent lamps
(a) Immunofluorescence staining of p-H2AX in UV-irradiated mouse skin and tumors. Keratin 14 was used as counterstain. Scale bar is 100μm. (b) Quantification of p-H2AX staining, n=6, 8 and 4 for WT skin, Smad4 −/− skin, Smad4 −/− tumors, respectively. (c) Alkaline comet assay using WT and Smad4 <t>−/−</t> <t>keratinocytes.</t> (d) Quantification of % DNA in comet tail. n=44. (e) CPD levels in <t>UVB-irradiated</t> WT and Smad4 −/− keratinocytes was measured by ELISA. CPD level in Smad4 −/− keratinocytes at 10 minutes was set at 100%. P ≥ 0.1 at 5, 10 and 30 minutes, respectively. *denotes p ≤ 0.0002, and ** denotes p ≤ 0.003, n=3. Error bars are S.E.M. (all panels).
Tl12 Fluorescent Lamps, supplied by Philips Healthcare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Philips Healthcare uvb
(a) Immunofluorescence staining of p-H2AX in UV-irradiated mouse skin and tumors. Keratin 14 was used as counterstain. Scale bar is 100μm. (b) Quantification of p-H2AX staining, n=6, 8 and 4 for WT skin, Smad4 −/− skin, Smad4 −/− tumors, respectively. (c) Alkaline comet assay using WT and Smad4 <t>−/−</t> <t>keratinocytes.</t> (d) Quantification of % DNA in comet tail. n=44. (e) CPD levels in <t>UVB-irradiated</t> WT and Smad4 −/− keratinocytes was measured by ELISA. CPD level in Smad4 −/− keratinocytes at 10 minutes was set at 100%. P ≥ 0.1 at 5, 10 and 30 minutes, respectively. *denotes p ≤ 0.0002, and ** denotes p ≤ 0.003, n=3. Error bars are S.E.M. (all panels).
Uvb, supplied by Philips Healthcare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/uvb/product/Philips Healthcare
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Beiersdorf Inc broad spectrum (uva and uvb) spf 30 sunscreen nivea sun
(a) Immunofluorescence staining of p-H2AX in UV-irradiated mouse skin and tumors. Keratin 14 was used as counterstain. Scale bar is 100μm. (b) Quantification of p-H2AX staining, n=6, 8 and 4 for WT skin, Smad4 −/− skin, Smad4 −/− tumors, respectively. (c) Alkaline comet assay using WT and Smad4 <t>−/−</t> <t>keratinocytes.</t> (d) Quantification of % DNA in comet tail. n=44. (e) CPD levels in <t>UVB-irradiated</t> WT and Smad4 −/− keratinocytes was measured by ELISA. CPD level in Smad4 −/− keratinocytes at 10 minutes was set at 100%. P ≥ 0.1 at 5, 10 and 30 minutes, respectively. *denotes p ≤ 0.0002, and ** denotes p ≤ 0.003, n=3. Error bars are S.E.M. (all panels).
Broad Spectrum (Uva And Uvb) Spf 30 Sunscreen Nivea Sun, supplied by Beiersdorf Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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RUVBL1 Antibody raised in Rabbit validated in WB IHC P Flow in Human
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Image Search Results


(a) Immunofluorescence staining of p-H2AX in UV-irradiated mouse skin and tumors. Keratin 14 was used as counterstain. Scale bar is 100μm. (b) Quantification of p-H2AX staining, n=6, 8 and 4 for WT skin, Smad4 −/− skin, Smad4 −/− tumors, respectively. (c) Alkaline comet assay using WT and Smad4 −/− keratinocytes. (d) Quantification of % DNA in comet tail. n=44. (e) CPD levels in UVB-irradiated WT and Smad4 −/− keratinocytes was measured by ELISA. CPD level in Smad4 −/− keratinocytes at 10 minutes was set at 100%. P ≥ 0.1 at 5, 10 and 30 minutes, respectively. *denotes p ≤ 0.0002, and ** denotes p ≤ 0.003, n=3. Error bars are S.E.M. (all panels).

Journal: The Journal of investigative dermatology

Article Title: Smad4 loss in mouse keratinocytes leads to increased susceptibility to UV carcinogenesis with reduced Ercc1-mediated DNA repair

doi: 10.1038/jid.2013.213

Figure Lengend Snippet: (a) Immunofluorescence staining of p-H2AX in UV-irradiated mouse skin and tumors. Keratin 14 was used as counterstain. Scale bar is 100μm. (b) Quantification of p-H2AX staining, n=6, 8 and 4 for WT skin, Smad4 −/− skin, Smad4 −/− tumors, respectively. (c) Alkaline comet assay using WT and Smad4 −/− keratinocytes. (d) Quantification of % DNA in comet tail. n=44. (e) CPD levels in UVB-irradiated WT and Smad4 −/− keratinocytes was measured by ELISA. CPD level in Smad4 −/− keratinocytes at 10 minutes was set at 100%. P ≥ 0.1 at 5, 10 and 30 minutes, respectively. *denotes p ≤ 0.0002, and ** denotes p ≤ 0.003, n=3. Error bars are S.E.M. (all panels).

Article Snippet: Keratinocytes in culture were irradiated with 60 mJ/cm 2 of UVB, culture medium (Lonza, Switzerland) was replaced with fresh medium and cells were incubated in a 5% CO 2 incubator for the indicated time period.

Techniques: Immunofluorescence, Staining, Irradiation, Alkaline Single Cell Gel Electrophoresis, Enzyme-linked Immunosorbent Assay

(a) Snai1 mRNA level in UV-irradiated keratinocytes, the level in untreated WT keratinocytes was set at 1. (b) Snai1 mRNA level in UV-irradiated mice skin, the level in UV-irradiated WT skin was set at 1, n=4 for WT , n=7 for Smad4 −/− . * denotes p= 0.027 (c) Ercc1 mRNA level in human Snai1 -transfected keratinocytes, the level in empty vector transfected WT keratinocytes was set at 1. ** denotes p≤ 0.002. (d) Snail Chromatin Immunoprecipitation from WT and Smad4 −/− keratinocyte extracts with/without UV irradiation. * p<0.03, ** p< 0.005. (e) CPD level in UVB-irradiated Snai1 transfected keratinocytes *p = 5.09E −5 , ** p=0.005, *** p=0.01. (f) CPD level in UVB-irradiated Ercc1 transfected keratinocytes, *=0.026, **=0.046, *** p=0.10, n=3. Error bars: S.E.M.

Journal: The Journal of investigative dermatology

Article Title: Smad4 loss in mouse keratinocytes leads to increased susceptibility to UV carcinogenesis with reduced Ercc1-mediated DNA repair

doi: 10.1038/jid.2013.213

Figure Lengend Snippet: (a) Snai1 mRNA level in UV-irradiated keratinocytes, the level in untreated WT keratinocytes was set at 1. (b) Snai1 mRNA level in UV-irradiated mice skin, the level in UV-irradiated WT skin was set at 1, n=4 for WT , n=7 for Smad4 −/− . * denotes p= 0.027 (c) Ercc1 mRNA level in human Snai1 -transfected keratinocytes, the level in empty vector transfected WT keratinocytes was set at 1. ** denotes p≤ 0.002. (d) Snail Chromatin Immunoprecipitation from WT and Smad4 −/− keratinocyte extracts with/without UV irradiation. * p<0.03, ** p< 0.005. (e) CPD level in UVB-irradiated Snai1 transfected keratinocytes *p = 5.09E −5 , ** p=0.005, *** p=0.01. (f) CPD level in UVB-irradiated Ercc1 transfected keratinocytes, *=0.026, **=0.046, *** p=0.10, n=3. Error bars: S.E.M.

Article Snippet: Keratinocytes in culture were irradiated with 60 mJ/cm 2 of UVB, culture medium (Lonza, Switzerland) was replaced with fresh medium and cells were incubated in a 5% CO 2 incubator for the indicated time period.

Techniques: Irradiation, Transfection, Plasmid Preparation, Chromatin Immunoprecipitation